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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Influence of Serum Concentration on tPA Production of CHO Cell) ผู้เขียน: Teerapatr Srinorakutara, Jin-Ho Jang, Mutsumi Takagi, Toshiomi Yoshida สื่อสิ่งพิมพ์:pdf AbstractThe effect of initial serum concentration on cell growth, and the production of tissue plasminogen activator (tPA) is investigated across batch fermentation of a Chinese hamster ovary (CHO) growing in newborn calf serum (NCS) medium. It was found that initial serum concentration influenced on CHO cell growth rate and cell yield. At high-serum content, the uptake rate of glucose decreased although the specific growth rate was higher, indicating a serum component becoming growth limiting. An average glucose uptake rate in 10% (v/v) NCS medium was 13.84 mg/106 cells/h as in 1% (v/v) NCS medium was 18.17 mg/106 cells/h. It was also found that the tPA or the maximum tPA productions increased with initial serum concentration. At 7.12% NCS, the maximum tPA production gave the highest value (5.55 mg/l). The effect of initial serum concentration on the main product was similar to that of major by-product (e.g. lactate and ammonia). |
 ผลงานตีพิมพ์ในวารสารวิชาการSearch for aflatoxin and trichothecene production inhibitors and analysis of their modes of actionผู้แต่ง: Dr.Usuma Jermnak, Associate Professor , Shohei Sakuda, Tomoya Yoshinari, Tomohiro Furukawa, Keiko Takagi, Kurin Iimura, Toshiyoshi Yamamoto, Michio Suzuki, Hiromichi Nagasawa, วารสาร:   
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 Researcherดร. สหภพ ดอกแก้วที่ทำงาน:ภาควิชาเพาะเลี้ยงสัตว์น้ำ คณะประมง สาขาที่สนใจ:Marine Aquarium, เพาะเลี้ยงชายฝั่ง , สัตว์น้ำสวยงาม Resume |
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